|
Hello, in my 3D simulation cells shall move (chemotact) due to a gradient of secreted ATTR. They actually move, but simultaneously dissolve into small peaces, which disappear completely after a certain time. I read about some plugins concerning connectivity management but they only work in 2D. My cell settings are: TargetVolume = 120; LambdaVolume = 50; TargetSurface = 80; LambdaSurface = 50.0 Contacts: Medium <-> Cell = 150; Cell <-> Cell = 1 ATTR settings are: Diffusion constant = 0.15; Decay constant = 0.000; Secretion = 2000 Chemotaxis: Lambda = 1000 As you see, I tried to minimize chances for cells to have too much surface or medium <-> cell contact. But this didn't help. What is the key to let cells (in 3d) stay connected just like in the 2d bacterium/macrophage example? That is: Cells stay connected and compact instead of breaking in parts |
|
Hello, What is the value of the temperature you are using? I have four suggestions. 1) Try to make the two adhesion energies more close to each other ; 2) Try using a diffusion constant equal or smaller than 0.1 ; 3) Try using a weaker chemotaxis strength or a smaller secretion rate ; 4) Start using a non-zero decay constant (about 10x smaller than the diffusion rate), otherwise the ATTR levels will become to high. Dimensions 100 * 100 * 100 Anneal = 0 Steps 10000 Temperature = 15 Flip2DimRatio = 1.0 Thank you, I will try to apply these changes and report about the result!
(Jan 18 at 03:49)
Apotekarnes
|
|
also take a look at this page for param. selection: http://glazier.biocomplexity.indiana.edu:8888/AshiriniWiki/CC3D/How_to_Simulate_Using_CC3D |
Any ideas? The issue is quite urgent ;)